While the congregation has several anniversary events planned for the coming year, last week there were special services in honour of the milestone.
The Rev John Woodside, from Drogheda, was the invited preacher for evening services of thanksgiving and reflection held on Wednesday and Friday.
And the Moderator of the Presbyterian Church in Ireland, the Right Reverend Dr John Finlay, visited the congregation and conducted morning worship on Sunday.
The evening service saw the return of the Rev Philip McKelvey, a former assistant minister within the congregation.
As a congregation within the Presbyterian family, Kilfennan is rather young although its congregational was formed out of the existing Presbyterian congregation in Great James Street.
This congregation itself was opened in May 1837 and was built to quote from the Session Minutes "as a result of the fighting seats for the services at First Derry Congregation".
It was somewhat hard for the Congregation to accept that a church built to serve the needs of Presbyterians in the city would 145 years later have to close.
Changing demographics within the city which were not unrelated to the Troubles left an aging congregation with a significant decline and marriages and baptisms.
Kilfennan's theme for the 25th anniversary is "Pressing Onward" - looking forward to the next 25 years just as another generation 25 years ago were looking forward with faith and expectation as they left Great james Street for the Waterside area.
Mr TS Mooney, editor of the church magazine, wrote in the final edition that the congregation of Great James were akin to the children of Israel crossing the River Jordan.
"We were fortunate not to have to wait for a parting of the waters but were able to use the Craigavon Bridge. As the vast majority of the congregation were living on the Waterside history was repeating itself as the church building was opened to be near the people of God.
"We held our last service on Great James Street on September 5 1982 and it had to be held in the Church Hall.
"For the pulpit, Communion Table and Baptismal Font had already been transferred to the new church-a constant reminders of our link with our past history.
"So with our new minister Reverend Edgar McKinney (newly installed on April 6, 1982] Great James Street 'ported' (Presbyterian technical language for moving to a new location] to become Kilfennan Presbyterian.
"It was with some trepidation that we made the move: believing that it was God's will that we should serve Him in our new church. The new church building was opened and dedicated on September 13 1982 by the Very reverend Dr John Girvan, deputising for the then Moderator who was ill on the day.
"Some memories of that day: Dr Girvan remarking that both he and we were unique in this respect; that his name was on both the foundation and opening stones of our church for he had performed both duties for us. He also assured us that although the church was very full on the opening day it would be some time before we had that number again.
"Since Kilfennan was and is a multi-purpose building we have no pews and so we purchased 150 chairs. We borrowed 200 chairs from a local school for the opening, not expecting a higher number for the opening services.
"God, however, knew better than us: an to our joy we had to purchase 200 chairs for a growing congregation.
"Further evidence that it has been right to move was soon found in our Sunday School which grew from 40 to some 300 within the first year.
"Kilfennan Presbyterian continues to serve the community after 25 years with our member families almost four times that of Great James Street. As a congregation we seek through our various organisations and events which are open to all. Through a variety of organisations we seek to provide opportunities for recreation and fellowship; we have a vibrant youth programme served and staffed by a team of willing volunteers.
"In recent years we have added to our youth programme and event called 'Somewhere Else' -which has hosted a variety of Christian bands seeking to convey the Gospel in a medium accessible to many young people. The highlight of this programme has been an open air concert in the grounds of the church which has drawn 800-900 people.
"Other examples of our community involvement include our Mothers and Toddlers Group, Our Men's Club (for the retired/unemployed) and our Ministry to the Elderly.
"The latter is staffed by a core of volunteers who take it in turns to visit one of the Residential Homes on the waterside where every Sunday they conducted a short service at the traditional time for a Sunday Service-11.30am.
"To rephrase a Bible Verse "A Church set on a hill cannot be a hid." Our church is in a elevated position with magnificent views of the City and hills beyond. It is our hope and prayer that we have been shining the Light of Christ for the last 25 years and for many more to come.
"Our Minister, Reverend Rob Craig, Session and Congregation strive to meet the needs of a changing society where the secular vies to replace the spiritual in so many ways.
"We pray that, with God's help, we in the future can build on our service to Him over the last 25 and 170 years.
"You are welcome to join with us in celebrating our silver anniversary."